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Image Search Results
Journal: PLoS ONE
Article Title: SLC37A1 and SLC37A2 Are Phosphate-Linked, Glucose-6-Phosphate Antiporters
doi: 10.1371/journal.pone.0023157
Figure Lengend Snippet: Double immunofluorescence staining for SLC37A1, SLC37A2, SLC37A3, or G6PT (red fluorescence), endogenous calreticulin (green fluorescence), and DAPI in COS-1 cells infected with Ad-SLC37A1-5Flag, Ad-SLC37A2-5Flag, Ad-SLC37A3-5Flag, or Ad-G6PT-5Flag as described under . Co-localization of SLC37 proteins with the ER-marker calreticulin is indicated by yellow in the merged image.
Article Snippet: The
Techniques: Double Immunofluorescence Staining, Fluorescence, Infection, Marker
Journal: PLoS ONE
Article Title: SLC37A1 and SLC37A2 Are Phosphate-Linked, Glucose-6-Phosphate Antiporters
doi: 10.1371/journal.pone.0023157
Figure Lengend Snippet: Microsomes were isolated from COS-1 cells infected with Ad-SLC37A1-5Flag, Ad-SLC37A2-5Flag, Ad-SLC37A3-5Flag or Ad-G6PT and proteoliposomes were reconstituted from the respective detergent solubilized microsomal membrane extracts as described under . Results shown are from three independent experiments, each point determined in triplicate. (A) Western-blot analysis of SLC37 proteins during reconstitution of proteoliposomes from the respective microsomal membranes using a mouse monoclonal antibody against the Flag epitope or a rabbit polyclonal antibody against G6PT . Each lane contains 10 µg protein. (B) G6P or P i uptake in 50 mM P i -loaded SLC37A1-, SLC37A2-, SLC37A3-proteoliposomes. G6P or P i uptake in 50 mM P i -loaded G6PT- and mock-proteoliposomes were used as controls.
Article Snippet: The
Techniques: Isolation, Infection, Membrane, Western Blot, FLAG-tag
Journal: PLoS ONE
Article Title: SLC37A1 and SLC37A2 Are Phosphate-Linked, Glucose-6-Phosphate Antiporters
doi: 10.1371/journal.pone.0023157
Figure Lengend Snippet: Proteoliposomes were reconstituted from detergent solubilized microsomal membrane extracts isolated from Ad-SLC37A1-5Flag, Ad-SLC37A2-5Flag, or Ad-G6PT infected COS-1 cells as described under . Results shown are from three independent experiments, each point determined in triplicate. (A) Effects of P i -loading on G6P or P i uptake activity of SLC37 proteoliposomes. The concentrations of P i loaded were 50 mM, 20 mM, and 0 mM (MOPS). (B) Inhibition of G6P uptake in 50 mM P i -loaded SLC37 proteoliposomes by external P i or inhibition of P i uptake in 50 mM P i -loaded SLC37 proteoliposomes by external G6P. (C) Effects of vanadate on G6P and P i uptake activities of SLC37 proteoliposomes. Data are presented as the mean ± SEM. * p <0.05; ** p <0.005.
Article Snippet: The
Techniques: Membrane, Isolation, Infection, Activity Assay, Inhibition
Journal: PLoS ONE
Article Title: SLC37A1 and SLC37A2 Are Phosphate-Linked, Glucose-6-Phosphate Antiporters
doi: 10.1371/journal.pone.0023157
Figure Lengend Snippet: The mRNA levels of SLC37 members, relative to G6PT/SLC37A4, in mouse liver, kidney, intestine, and pancreas.
Article Snippet: The
Techniques:
Journal: PLoS ONE
Article Title: SLC37A1 and SLC37A2 Are Phosphate-Linked, Glucose-6-Phosphate Antiporters
doi: 10.1371/journal.pone.0023157
Figure Lengend Snippet: Proteoliposomes were reconstituted from detergent solubilized microsomal membrane extracts expressing SLC37A1, or SLC37A2 and were loaded with 50 mM P i as described under . The 50 mM P i -loaded G6PT proteoliposomes were used as a control. Results shown are from three independent experiments, each point determined in triplicate. Data are presented as the mean ± SEM. ** p <0.005.
Article Snippet: The
Techniques: Membrane, Expressing, Control
Journal: PLoS ONE
Article Title: SLC37A1 and SLC37A2 Are Phosphate-Linked, Glucose-6-Phosphate Antiporters
doi: 10.1371/journal.pone.0023157
Figure Lengend Snippet: Microsomal membranes were isolated from COS-1 cells infected with Ad-G6Pase-α, Ad-G6PT, Ad-SLC37A1-5Flag, or Ad-SLC37A2-5Flag, co-infected with Ad-G6PT/Ad-G6Pase-α, Ad-SLC37A1-5Flag/Ad-G6Pase-α, or Ad-SLC37A2-5Flag/Ad-G6Pase-α, or co-infected with Ad-G6PT/Ad-G6Pase-β, Ad-SLC37A1-5Flag/Ad-G6Pase-β, or Ad-SLC37A2-5Flag/Ad-G6Pase-β as described in . Results represent three independent experiments, with each data point determined in triplicate. Data are presented as the mean ± SEM. ** p <0.005.
Article Snippet: The
Techniques: Isolation, Infection